Back to results
US 11,952,586 B2Granted
B-cell cultivation method
AssigneeHoffmann-La Roche Inc.
InventorsSonja Offner, Friederike Jung
Life of this patent
Estimating the term…
Abstract
Herein is reported a method for the co-cultivation of single deposited B-cells, which can be of any source, with EL-4 B5 feeder cells in a suitable co-cultivation medium. In the herein reported methods the EL-4 B5 cells have been irradiated with a dose of less than 40 Gy, preferably 9.5 Gy or less. Thereby the EL-4 B5 cells have a higher viability and maintain the ability to divide in cultivation at doses less than 6 Gy.
Claims20
A method for cultivating one or more B-cells comprising the step of co-cultivating one or more B-cells with EL-4 B5 cells,
- wherein the EL-4 B5 cells have been irradiated prior to the co-cultivation with a dose of 9.5 Gy or less but greater than 0 Gy, and
- wherein the number of EL-4 B5 cells at the start of the co-cultivating is less than 5×104 per B-cell.
Dependencies are inferred from the claim text.