Interfering with HD-Zip transcription factor repression of gene expression to produce plants with enhanced traits
AssigneeMonsanto Technology LLC
InventorsCara L. Griffith, Abha Khandelwal, Paul J. Loida, Elena A. Rice, Rebecca L. Thompson, Sivalinganna Manjunath
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Abstract
A recombinant DNA construct is disclosed. When the recombinant DNA construct is expressed in a plant or a plant cell, endogenous HD-Zip class II proteins become less able to repress DNA transcription of the genes they typically regulate. The recombinant DNA construct can be expressed in plant cells to produce plants with enhanced phenotypes. Methods of making transgenic plants comprising the recombinant DNA construct, and plants produced thereby are also disclosed.
Claims8
A recombinant DNA construct comprising a DNA molecule that encodes a N-terminal truncation variant of a HD-Zip class II transcription factor protein that has an amino acid sequence with at least 95% amino acid sequence identity with the full-length amino acid sequence of SEQ ID NO:
- 113, and operably linked to a heterologous promoter, wherein the N-terminal truncation variant is missing the N-terminal amino acid portion comprising amino acid position 1 to amino acid position 59 of the HD-Zip class II amino acid sequence transcription factor protein relative to the amino acid sequence of SEQ ID NO:
- 26, and wherein said N-terminal truncation variant of the HD-Zip class II transcription factor protein has a loss-of-function mutation in a transcriptional repression domain.
Dependencies are inferred from the claim text.