Compositions and methods for site directed genomic modification
AssigneeMonsanto Technology LLC
InventorsBrent Brower-Toland, Andrei Y. Kouranov, Rosemarie Kuehn, Richard J. Lawrence, Ervin D. Nagy, Linda Rymarquis, Veena Veena
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Abstract
The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.
Claims14
A recombinant DNA construct comprising a 7SL promoter;
- operably linked to:
- (i) a sequence encoding a single-guide RNA (sgRNA), or
- (ii) a sequence specifying a non-coding RNA;
- and wherein the sequence of said 7SL promotor comprises SEQ ID NO:174;
- or a fragment thereof, wherein the fragment is at least 140 bp in length, wherein the 7SL promotor or fragment thereof and the sgRNA or non-coding RNA come from different sources.
Dependencies are inferred from the claim text.