Device and methods for delivery of biphasic electrical pulses for non-thermal ablation

A device comprising:

• a generator programmed to provide a plurality of predetermined electrical treatment protocols, with at least one of the treatment protocols providing for:
• a plurality of bipolar electrical pulses;
• delivery of the bipolar electrical pulses at a voltage of up to 5,000 volts;
• delivery of the bipolar electrical pulses at a frequency of up to about 500 kHz;
• and
• delivery of the bipolar electrical pulses at a pulse length of at least 10 ns to 1 millisecond;
• wherein the generator is configured to be operatively coupled to at least two electrodes;
• wherein the bipolar electrical pulses:
• comprise a first polarity portion and a second polarity portion, each portion having a square waveform;
• are configured to alternate polarity during pulse delivery from the first polarity portion of one polarity to the second polarity portion of the opposite polarity;
• are configured with a delay between the first polarity portion and the second polarity portion, wherein the delay is from 1 times the pulse length to 5 times the pulse length;
• and
• are configured to non-thermally ablate cells of a target area.

A method comprising:

• selecting an electrical treatment protocol from a series of electrical treatment protocol options provided by a generator programmed with the electrical treatment protocol options;
• coupling the generator to at least two electrodes;
• activating the generator to apply the selected electrical treatment protocol, wherein the selected electrical treatment protocol comprises a plurality of bipolar electrical pulses comprising a voltage of up to 5,000 volts and a frequency of about 500 kHz;
• wherein the plurality of bipolar electrical pulses of the selected electrical treatment protocol:
• comprises a first polarity portion and a second polarity portion, each portion being of opposite polarity from the other and having a square waveform;
• is configured to alternate polarity from the first polarity portion to the second polarity portion separated by a delay during pulse delivery such that the first polarity portion has a first polarity before the delay and the second polarity portion has an opposite polarity after the delay, wherein the delay is from 1 times a pulse length to 5 times a pulse length;
• and
• is configured to non-thermally ablate cells of a target area.