Closed cell culturing and harvesting system
AssigneeShanghai Longevity Co., Ltd.
InventorsTian Xia, Ke Ma
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Abstract
A bioreactor includes a base and a lid. The base includes two opposing curved or convoluted surfaces, two opposing flat surfaces, a window disposed on one of the flat surfaces, the window having a higher degree of transparency compared to other portions of the base, wherein images or videos of cells are captured through the window by non-invasive ISM device, and a rounded bottom. The lid is attachable to the base. The lid includes a shaft and a semipermeable membrane attached to the shaft, the semipermeable membrane being permeable to oxygen but impermeable to viruses and bacteria. The PAT-based online analysis directs the adaptive manipulations of bioreactor towards efficient, automated, and GMP-compliant clinical protocols of cell culture.
Claims20
A bioreactor configured to culture cells, the bioreactor comprising a bottom, side surfaces disposed above the bottom, and a lid disposed above the side surfaces, the side surfaces comprising:
- two opposing curved or convoluted surfaces;
- two opposing flat surfaces;
- and
- windows disposed on the respective flat surfaces, the windows having a higher degree of transparency compared to other portions of the bottom and the side surfaces, wherein images or videos of cells are captured through the windows by an imaging device placed against a window;
- and
- the lid comprising a connector attachable to an external device.
A method of capturing images or videos of cells, the method comprising:
- culturing cells within a bioreactor, wherein the bioreactor comprises a bottom, side surfaces disposed above the bottom, and a lid disposed above the side surfaces, the side surfaces comprising:
- two opposing curved or convoluted surfaces;
- two opposing flat surfaces;
- and
- windows disposed on the respective flat surfaces, the windows having a higher degree of transparency compared to other portions of the bottom and the side surfaces;
- positioning an imaging device against a window of the windows;
- and
- capturing the images or the videos of the cells through the window.
Dependencies are inferred from the claim text.