Subtilase variants
AssigneeNovozymes A/S
InventorsMiguel Duarte Guilherme Pereira Toscano, Esben Peter Friis, Rolf Thomas Lenhard, Signe Eskildsen Larsen, Astrid Munch, Bena-Marie Lue, Mikael Bauer
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Abstract
The present invention relates to novel protease variants exhibiting improved stability and or improved wash performance in liquid detergent. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.
Claims16
A subtilase variant having at least 90% sequence identity to SEQ ID NO:
- 3 wherein the variant has a glutamic acid residue (E) in position 101, wherein the subtilase variant further comprises one or more substitutions selected from the group consisting of S3T, R45E,D,Q, P55N, T58W,Y,L, Q59D,M,N,T, G61D,R, S87E, G97S, A98D,E,R, S106A,W, N117E, H120V,D,K,N, S124M, P129D, E136Q, Q137H, S143W, S156D, S161T, S163A,G, Y171L, A172S, N185Q, V199M, Y209W, M222Q, N238H, V244T, N261T,D and L262N,Q,D,E, wherein the variant has increased stability in a liquid detergent composition compared to the subtilase having the amino acid sequence of SEQ ID NO:
- 3 and wherein the positions corresponds to the positions of SEQ ID NO:
- 1.
Dependencies are inferred from the claim text.