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US 11,952,557 B2Granted
Alpha-amylase variants and polynucleotides encoding same
AssigneeNovozymes A/S
InventorsCarsten Andersen, Chakshusmathi Ghadiyaram, Rajendra Kulothungan Sainathan, Padmavathi Balumuri, Padma Venkatachalam Iyer, Iben Damager, Astrid Munch, Sohel Dalal
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Abstract
The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.
Claims27
A variant of a parent alpha-amylase having alpha-amylase activity, which:
- a) is a polypeptide having at least 85% sequence identity to SEQ ID NO:
- 13 or SEQ ID NO:
- 14, and
- b) comprises a substitution or a deletion at a position corresponding to A174 of the amino acid sequence as set forth in SEQ ID NO:
- 13 or SEQ ID NO:
- 14, and comprises a substitution and/or deletion in two, three or four positions corresponding to positions R181, G182, D183, and G184 of the amino acid sequence as set forth in SEQ ID NO:
- 13 or SEQ ID NO:
- 14,
- c) has alpha-amylase activity, and
- d) has an improved wash performance at a low temperature of 5° C. to 40° C., wherein the improved wash performance is an Improvement Factor (IF) of >1, when compared to the parent alpha-amylase.
7 more elements
Dependencies are inferred from the claim text.