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US 11,952,433 B2Granted
Heterochiral peptide-complex and composition for measuring nuclear magnetic resonance spectroscopy residual dipolar coupling containing self-assembled intermediates of heterochiral peptide-complex
AssigneeIndustry-Academic Cooperation Foundation, Yonsei University
InventorsYong-Beom Lim, Hye Soo Lee
Life of this patent
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Abstract
The present disclosure relates to a heterochiral peptide complex, a self-assembled intermediate thereof, an alignment medium composition used for nuclear magnetic resonance spectroscopy residual dipolar coupling (NMR-RDC) measurement, which includes the same, and a method for NMR measurement for a biomolecule. Since the heterochiral peptide complex contains a β-sheet peptide and has superior structural stability against environmental factors such as heat, acidity and ionic strength, it is applicable as an alignment medium for measuring RDC for various biomolecules.
Claims9
A method for analyzing an NMR spectrum of a biomolecule, comprising:
- (a) a step of mixing a peptide complex in a mixture solvent;
- (b) a step of adding a biomolecule to be measured in the mixture solution;
- and
- (c) a step of conducting NMR measurement for the mixture of the biomolecule and the peptide complex, wherein the peptide complex comprises a peptide represented by General Formula 1 or 2, comprising a heterochiral peptide;
- and a compound represented by Chemical Formula 1, covalently bound to N-terminal of the peptide:
- [Y1]a-{-[X1]b-[Y2]c}d-[X2]e [General Formula 1]
- [X1]r{-[Y1]g-[X2]h}i-[Y2]j [General Formula 2]
- wherein
- each of X1 and X2, which are identical to or different from each other, is independently an amino acid selected from D-Trp, D-Phe, D-Tyr, D-Val, D-Leu and D-Ile,
- each of Y1 and Y2, which are identical to or different from each other, is independently an amino acid selected from L-Trp, L-Phe, L-Tyr, L-Val, L-Leu and L-Ile, and
- each of a, f, e and j is independently an integer selected from 0 to 2, each of b, c, g and h is independently an integer selected from 1 to 5, each of d and i is independently an integer selected from 1 to 10,
- wherein each of n and m is independently an integer selected from 1 to 12,
- wherein the peptide represented by General Formula 1 or General Formula II comprises at least five amino acid residues,
- wherein the amino acid in position 3, relative to the N-terminal of the peptide, is selected from the group consisting of D-Trp, D-Phe, D-Tyr, D-Val, D-Leu, D-Ile, L-Trp, L-Tyr, L-Val, L-Leu and L-Ile.
11 more elements
A method for analyzing an NMR spectrum of a biomolecule, comprising:
- (a) a step of mixing a peptide complex in a mixture solvent;
- (b) a step of adding a biomolecule to be measured in the mixture solution;
- and
- (c) a step of conducting NMR measurement for the mixture of the biomolecule and the peptide complex, wherein the peptide complex comprises a peptide represented by General Formula 1 or 2, comprising a heterochiral peptide;
- and a compound represented by Chemical Formula 1, covalently bound to N-terminal of the peptide:
- [Y1]a-{-[X1]b-[Y2]c}d-[X2]e [General Formula 1]
- [X1]f{-[Y1]g-[X2]h}i-[Y2]j [General Formula 2]
- wherein
- each of X1 and X2, which are identical to or different from each other, is independently an amino acid selected from D-Trp, D-Phe, D-Tyr, D-Val, D-Leu and D-Ile,
- each of Y1 and Y2, which are identical to or different from each other, is independently an amino acid selected from L-Trp, L-Phe, L-Tyr, L-Val, L-Leu and L-Ile,
- each of a, f, e and j is independently an integer selected from 0 to 2, each of b, c, g and h is independently an integer selected from 1 to 5, each of d and i is independently an integer selected from 1 to 10,
- wherein each of n and m is independently an integer selected from 1 to 12, and
- wherein the peptide is any one selected from the heterochiral peptides represented by SEQ ID NOS:
- 1 to 12:
- (D)Trp-(L)Trp-(D)Trp-(L)Trp-(D)Trp-(L)Trp [SEQ ID NO:
- 1]
- (D)Val-(L)Val-(D)Val-(L)Val-(D)Val-(L)Val [SEQ ID NO:
- 2]
- (D)Phe-(L)Phe-(D)Phe-(L)Phe-(D)Phe-(L)Phe [SEQ ID NO:
- 3]
- (L)Trp-(D)Trp-(L)Trp-(D)Trp-(L)Trp-(D)Trp [SEQ ID NO:
- 4]
- (L)Val-(D)Val-(L)Val-(D)Val-(L)Val-(D)Val [SEQ ID NO:
- 5]
- (L)Phe-(D)Phe-(L)Phe-(D)Phe-(L)Phe-(D)Phe [SEQ ID NO:
- 6]
- (L)Trp-(D)Trp-(L)Trp-(D)Trp-(L)Trp [SEQ ID NO:
- 7]
- (L)Val-(D)Val-(L)Val-(D)Val-(L)Val [SEQ ID NO:
- 8]
- (L)Phe-(D)Phe-(L)Phe-(D)Phe-(L)Phe [SEQ ID NO:
- 9]
- (D)Trp-(L)Trp-(D)Trp-(L)Trp-(D)Trp [SEQ ID NO:
- 10]
- (D)Val-(L)Val-(D)Val-(L)Val-(D)Val [SEQ ID NO:
- 11]
- (D)Phe-(L)Phe-(D)Phe-(L)Phe-(D)Phe. [SEQ ID NO:
- 12]
35 more elements
Dependencies are inferred from the claim text.