Back to results
US 11,952,430 B2Granted
Multispecific antigen-binding molecules for cell targeting and uses thereof
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsLauric Haber, Jennifer A. Finney, Ryan McKay, Eric Smith, Chia-Yang Lin
Life of this patent
Estimating the term…
Abstract
The present invention provides multispecific antigen-binding molecules that bind both a T-cell antigen (e.g., CD3) and a target antigen (e.g., a tumor associated antigen, a viral or bacterial antigen), and which include a single polypeptide chain that is multivalent (e.g., bivalent) with respect to T-cell antigen binding, and uses thereof.
Claims24
A multispecific antigen-binding molecule, comprising:
- (a) a first polypeptide comprising, from N-terminus to C-terminus (i) a first antigen-binding domain that specifically binds human CD3, wherein the first antigen-binding domain comprises an immunoglobulin Fab domain comprising a heavy chain variable region (HCVR) and a heavy chain CH1 domain paired with a light chain variable region (LCVR) and a light chain CL domain;
- (ii) a first multimerizing domain comprising an immunoglobulin Fc domain;
- and (iii) a second antigen-binding domain that specifically binds human CD3, wherein the second antigen-binding domain is a single chain variable fragment (scFv) domain comprising a HCVR and a LCVR connected by a peptide linker;
- and
- (b) a second polypeptide comprising, from N-terminus to C-terminus (i) a third antigen-binding domain that specifically binds a target antigen, wherein the third antigen-binding domain comprises an immunoglobulin Fab domain comprising a HCVR and a heavy chain CH1 domain paired with a LCVR and a light chain CL domain;
- (ii) a second multimerizing domain comprising an immunoglobulin Fc domain;
- and (iii) a fourth antigen-binding domain that specifically binds a target antigen, wherein the fourth antigen-binding domain is a scFv domain comprising a HCVR and a LCVR connected by a peptide linker,
- wherein the first and the second multimerizing domains associate with one another to form the molecule.
5 more elements
Dependencies are inferred from the claim text.