HPV-specific binding molecules

A polynucleotide, comprising:

• (a) a nucleic acid sequence encoding a T cell receptor (TCR) or an antigen-binding portion thereof that binds to or recognizes a peptide epitope of human papillomavirus (HPV) 16 in the context of an MHC molecule, and
• (b) two or more homology arm(s) linked to the nucleic acid sequence, wherein:
• the two or more homology arms comprise a sequence homologous to one or more region(s) of an open reading frame of a T cell receptor alpha constant (TRAC) locus;
• wherein the two or more homology arm(s) comprises a 5′ homology arm and a 3′ homology arm,
• wherein the 5′ homology arm comprises the sequence set forth in SEQ ID NO:
• 1343, and
• wherein the 3′ homology arm comprises a sequence comprising at least 150 contiguous nucleotides of the sequence set forth in SEQ ID NO:
• 1344.