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AssigneeLanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences
InventorsHaixue Zheng, Fan Yang, Zixiang Zhu, Xiangle Zhang, Weijun Cao, Congcong Wang, Kangli Li, Huisheng Liu, Hong Tian, Keshan Zhang, Xiangtao Liu
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The present disclosure belongs to the technical field of biological products for veterinary medicine, and specifically relates to a recombinant foot-and-mouth disease virus (FMDV) with a reduced immunosuppressive activity, a preparation method and use thereof, and a recombinant vaccine strain. According to the present disclosure, it is firstly discovered that FMDV 3B protein has an immunosuppressive function, and key sites for exerting the immunosuppressive function are found. A recombinant FMDV vaccine strain with a lost immunosuppressive function in FMDV 3B protein is constructed by introducing amino acid mutations into three repeated copies of FMDV 3B protein.
A method for preparing a recombinant foot-and-mouth disease virus (FMDV), comprising mutating the amino acid at position 17 for each of 3B1, 3B2, and 3B3 of an FMDV 3B protein to glutamate to eliminate the immunosuppressive function of the FMDV 3B protein.
A recombinant FMDV, wherein the amino acid at position 17 for each of 3B1, 3B2, and 3B3 of an FMDV 3B protein is mutated to a polar amino acids using a gene mutation technology, thus eliminating the immunosuppressive function of the parental FMDV 3B protein.
A method for preparing a recombinant FMDV, comprising the following steps:
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