Chromatographic method for collecting blood coagulation factor VII with high yield
AssigneeKM Biologics Co., Ltd.
InventorsKentaro Ebisu, Takashi Kai, Nozomi Matsuo
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Abstract
A chromatographic method for collecting blood coagulation factor VII (Factor VII) and/or activated blood coagulation factor VII (activated Factor VII) from plasma-derived fractions with high yield is provided. In accordance with the method of the present invention, Factor VII and/or activated Factor VII of interest can be collected with a recovery rate of as high as 90% or more by letting Factor VII and/or activated Factor VII which fails to be adsorbed to a first anion exchange resin and remains in a non-adsorption fraction be adsorbed in a second anion exchange resin.
Claims5
A method for purifying blood coagulation factor VII (Factor VII) and activated blood coagulation factor VII (activated Factor VII) from cryoprecipitate-poor plasma-derived fraction, comprising the following steps:
- (a) applying cryoprecipitate-poor plasma-derived fraction to a first strong anion exchange resin;
- (b) adsorbing Factor VII and activated Factor VII from the cryoprecipitate-poor plasma-derived fraction to the first strong anion exchange resin and recovering a non-adsorbed fraction;
- (c) adsorbing Factor VII and activated Factor VII which remains in the non-adsorbed fraction in step (b) to a second strong anion exchange resin;
- and
- (d) recovering Factor VII and activated Factor VII from first and second strong anion exchange resins in steps (b) and (c), by elution wherein recovering Factor VII and activated Factor VII from the first strong anion exchange resin and second strong anion exchange resin occurs simultaneously.
Dependencies are inferred from the claim text.