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US 11,951,478 B2Granted
Microfluidic siphoning array for nucleic acid quantification
AssigneeCombinati Incorporated
InventorsJu-Sung Hung, Andrew Zayac, Megan Dueck
Life of this patent
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Abstract
In some aspects, the present disclose provides methods for amplifying and quantifying nucleic acids. Methods for amplifying and quantifying nucleic acids comprise isolating a sample comprising nucleic acid molecules into a plurality of microchambers, performing a polymerase chain reaction on the plurality of microchambers, and analyzing the results of the polymerase chain reaction. In some aspects, the present disclosure provides devices consistent with the methods herein.
Claims20
A method, comprising:
- filling a plurality of compartments of a microfluidic device with a reagent by applying said reagent at a first pressure to a microchannel of said microfluidic device, wherein said plurality of compartments are in fluid communication with said microchannel, and wherein said microfluidic device includes at least one gas permeable film or membrane covering said plurality of compartments;
- and
- transporting a first gas, within said plurality of compartments, to pass through said at least one gas permeable film or membrane covering said plurality of compartments by applying a second gas at a second pressure to said microchannel, wherein said second pressure is greater than said first pressure.
Dependencies are inferred from the claim text.