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US 11,951,476 B2Granted
Electronic-sensing and magnetic-modulation (ESMM) biosensor for phagocytosis quantification in pathogenic infections and methods of use thereof
AssigneeRutgers, The State University of New Jersey
InventorsUmer Hassan, Corey Norton
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Abstract
An electronic-sensing & magnetic-modulation (ESMM) biosensor device and methods of using the same, where the device incorporates electrical, microfluidic, and magnetic subsystems. The device and methods of using such a device can be applied to high throughput point-of-care screening for the quantification and evaluation of a subject's immune response to pathogenic infections, which can be useful for: early diagnosis, stratifying high-risk subjects infected with a pathogen; and determining the status or effectiveness of a therapeutic response.
Claims15
A method, comprising:
- applying a sample to a device,
- wherein the device comprises:
- a substrate;
- at least two electrodes;
- a microfluidic channel comprising at least one inlet and an outlet;
- and
- at least one magnet;
- wherein the at least two electrodes are disposed on the substrate,
- wherein the microfluidic channel is disposed in proximity to the at least two electrodes on the substrate,
- wherein the at least two electrodes are configured to generate an electric field, and
- wherein the at least one magnet is configured to provide a magnetic field in
- at least a portion of the microfluidic channel,
- wherein the sample comprises at least one cell;
- incorporating at least one magnetic particle to the sample;
- applying an input voltage of at least a single frequency to the at least two electrodes, generating an electric field;
- passing the sample from the at least one inlet to the outlet of the microfluidic channel through the electric field;
- a current signal from each of the at least two electrodesproducing a pulse;
- and detecting the pulse,
- wherein the pulse comprises:
- an absolute amplitude of 0 V-1 V;
- a pulse width of 0.02 ms-2 ms;
- a rise time of 0.01 ms −1 ms;
- a fall time of 0.01 ms −1 ms;
- a normalized width differential factor (NWDF) of −0.1-0.1;
- or combinations thereof.
22 more elements
A method, comprising:
- applying a sample to a device,
- wherein the device comprises:
- a substrate;
- at least two electrodes;
- a microfluidic channel comprising at least one inlet and an outlet;
- and
- at least one magnet;
- wherein the at least two electrodes are disposed on the substrate,
- wherein the microfluidic channel is disposed in proximity to the at least two electrodes on the substrate,
- wherein the at least two electrodes are configured to generate an electric field, and
- wherein the at least one magnet is configured to provide a magnetic field in at least a portion of the microfluidic channel,
- wherein the sample comprises at least one cell;
- incorporating at least one magnetic particle to the sample;
- applying an input voltage of at least a single frequency to the at least two electrodes, generating an electric field;
- passing the sample from the at least one inlet to the outlet of the microfluidic channel through the electric field;
- measuring a current signal from each of the at least two electrodes, producing a pulse;
- and
- detecting the pulse,
- wherein a pulse of the at least one cell of the sample with the at least one magnetic particle comprises an amplitude, a pulse width, a rise time, a fall time, a NWDF, or combinations thereof less than that in a pulse of a cell without at least one magnetic particle.
16 more elements
A method, comprising:
- applying a sample to a device,
- wherein the device comprises:
- a substrate;
- at least two electrodes;
- a microfluidic channel comprising at least one inlet and an outlet;
- and
- at least one magnet;
- wherein the at least two electrodes are disposed on the substrate,
- wherein the microfluidic channel is disposed in proximity to the at least two electrodes on the substrate,
- wherein the at least two electrodes are configured to generate an electric field, and
- wherein the at least one magnet is configured to provide a magnetic field in at least a portion of the microfluidic channel,
- wherein the sample comprises at least one cell;
- incorporating at least one magnetic particle to the sample;
- applying an input voltage of at least a single frequency to the at least two electrodes, generating an electric field;
- passing the sample from the at least one inlet to the outlet of the microfluidic channel through the electric field;
- measuring a current signal from each of the at least two electrodes, producing a pulse;
- and
- detecting the pulse,
- wherein a pulse of the at least one cell of the sample with the at least one magnetic particle comprises an amplitude, a pulse width, a rise time, a fall time, and a NWDF less than an amplitude, a pulse width, a rise time, a fall time, and a NWDF of a pulse of a cell without at least one magnetic particle.
16 more elements
Dependencies are inferred from the claim text.