Cryo formulation-based microneedle device for ocular delivery of bioactive therapeutic agents using a cryo-microneedle patch

A cryo formulation-based microneedle device for ocular delivery of bioactive therapeutic agents, comprising:

• one or more microneedle patches each including an array of miniaturized needles, each miniaturized needle defining a base end and a tip;
• and
• a substrate to which the base end of each miniaturized needle of the array of miniaturized needles is attached or integrated thereto;
• wherein the one or more microneedle patches are in a cryo status;
• wherein each of the one or more microneedle patches is adapted to be applied on a cornea of an eye, in which the miniaturized needles penetrate into the eye;
• wherein each of the one or more microneedle patches comprises a matrix solution, wherein the matrix solution comprises an aqueous base material and at least one cryoprotectant, the aqueous base solution includes glycerol, the cryoprotectant includes at least one of glycerol, sucrose, fructose, galactose, proteins, poly-l-lysine, starch, carboxymethyl cellulose (CMC), dextran, guar gum, pullulan, xanthan, xyloglucan, chondroitin, keratin and their derivatives thereof;
• and
• wherein the miniaturized needles are further arranged to melt so as to release one or more bioactive therapeutic agents into the eye to achieve a targeted therapeutic effect.

A cryo formulation-based microneedle device for ocular delivery of bioactive therapeutic agents, comprising:

• one or more microneedle patches each including an array of miniaturized needles, each miniaturized needle defining a base end and a tip;
• and
• a substrate to which the base end of each miniaturized needle of the array of miniaturized needles is attached or integrated thereto;
• wherein the one or more microneedle patches are in a cryo status;
• wherein each of the one or more microneedle patches is adapted to be applied on a cornea of an eye, in which the miniaturized needles penetrate into the eye;
• wherein each of the one or more microneedle patches comprises a matrix solution, wherein the matrix solution comprises glycerol, and wherein the amount of glycerol is from 1 to 5 wt. % of the matrix solution;
• and
• wherein the miniaturized needles are further arranged to melt so as to release one or more bioactive therapeutic agents into the eye to achieve a targeted therapeutic effect;
• and
• wherein the one or more bioactive therapeutic agents comprise predatory bacteria.