Acellular organs, and methods of producing the same
AssigneeAcro Biomedical Company. Ltd.
InventorsDar-Jen Hsieh, Chao-Yi Wei, Chao-Chin Chao, Jer-Cheng Kuo, Yi-Ping Lai, Srinivasan Periasamy
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Abstract
Provided are methods of producing an acellular organ. The method includes the steps of, subjecting an organ derived from an animal to a static supercritical fluid (SCF) treatment followed by a dynamic SCF treatment. Optionally, the method of the present disclosure further includes a hypertonic and a hypotonic treatments prior to the static SCF treatment, and/or a neutralizing treatment after the dynamic SCF treatment. Also disclosed herein are acellular organs produced by the present method.
Claims18
A method of producing an acellular organ, comprising:
- (a) subjecting an organ to a first supercritical fluid (SCF) in the presence of a first co-solvent under a first pressure of 200-500 bar at a first temperature between 30-50° C. for a first period of 10-100 minutes;
- and
- (b) subjecting the first SCF treated organ of the step (a) with a continuous flow of a second SCF in the presence of a second co-solvent under a second pressure of 200-500 bar at a second temperature between 30-50° C. for a second period of 10-100 minutes, wherein the flow rate of the second SCF is 10-30 liter per minute;
- wherein, the method does not comprise the step of treating the organ with an agent selected from the group consisting of an enzyme, an ion chelating agent, a detergent, a glycerol, and a combination thereof.
Dependencies are inferred from the claim text.