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US 11,951,211 B2Granted
DNA brick-assisted liposome sorting
AssigneeYale University
InventorsChenxiang Lin, Yang Yang
Life of this patent
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Abstract
A method for producing uniform-size liposomes is provided. The liposomes are coated with a sorting agent to yield a plurality of density-modified liposomes of different sizes. These liposomes are then separated using a densitometric method. The sorting agent includes both a density-modifying moiety and a targeting moiety.
Claims18
A method for producing uniform-size liposomes, the method comprising:
- coating a plurality of liposomes with a sorting agent to yield a plurality of density-modified liposomes of different sizes;
- separating the density-modified liposomes of different sizes using a densitometric method;
- and
- isolating one or more fractions of uniform-size liposomes,
- wherein the sorting agent comprises a density-modifying moiety and a targeting moiety, wherein the molecular mass of the density-modifying moiety is from 50 to 250 kDa, and
- wherein the liposomes within each of the one or more isolated fractions have a coefficient of variation of less than 15%.
3 more elements
A method for producing uniform-size liposomes, the method comprising:
- coating a plurality of liposomes with a sorting agent to yield a plurality of density-modified liposomes of different sizes;
- and
- separating the density-modified liposomes of different sizes using a densitometric method,
- wherein the sorting agent comprises a density-modifying moiety and a targeting moiety, wherein the molecular mass of the density-modifying moiety is from 50 to 250 kDa, and wherein the density-modifying moiety is a polynucleotide, and the method further comprising separating the density-modifying moiety from the targeting moiety using a nuclease after the separating step.
1 more element
A method for producing uniform-size liposomes, the method comprising:
- coating a plurality of liposomes with cholesterol-modified DNA oligonucleotides to yield a plurality of density-modified liposomes of different sizes;
- separating the density-modified liposomes of different sizes by isopycnic centrifugation, and
- isolating one or more fractions of uniform-size liposomes,
- wherein the cholesterol-modified DNA oligonucleotides form a six-helix-bundle rod of about 189 kD with a single cholesterol at the end of each DNA structure.
1 more element
A method for producing uniform-size liposomes, the method comprising:
- coating a plurality of liposomes with cholesterol-modified DNA oligonucleotides to yield a plurality of density-modified liposomes of different sizes;
- separating the density-modified liposomes of different sizes by isopycnic centrifugation, and
- isolating one or more fractions of uniform-size liposomes,
- wherein the cholesterol-modified DNA oligonucleotides form a three-pointed star of about 86 kD with a single cholesterol at the end of each DNA structure.
1 more element
Dependencies are inferred from the claim text.