Stabilization of glucagon by trehalose glycopolymer nanogels
AssigneeThe Regents of the University of California
InventorsHeather D. Maynard, Natalie Boehnke
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Abstract
Trehalose-based nanogels for stabilizing and controlled releasing biomolecules such as glucagons are disclosed. Specifically, trehalose-based nanogels comprise (a) a copolymer comprising first methacrylate units and second methacrylate units, wherein (i) the first methacrylate units comprise trehalose side chains; and (ii) the second methacrylate units comprise disulfide side chains; (b) dithiol cross-linkers; wherein the dithiol cross-linkers cross link the copolymer through the disulfide side chains of the second methacrylate units.
Claims17
A trehalose-based nanogel, the nanogel comprising:
- (a) a copolymer comprising first units and second units, wherein
- (i) the first units comprise trehalose side chains;
- and
- (ii) the second units comprise disulfide side chains;
- and
- (b) dithiol cross-linkers;
- wherein the dithiol cross-linkers cross-link the copolymer through the disulfide side chains of the second units;
- and
- wherein the nanogel further comprises one biomolecule covalently bonded with the copolymer,
- wherein the biomolecule is used as a cross-linker.
A Glucagon-containing nanogel, the nanogel comprising a trehalose-based copolymer comprising:
- a) a methacrylate-based backbone;
- b) at least one trehalose-based side chain;
- c) at least one disulfide side chain;
- and
- d) glucagons;
- wherein the glucagons are chemically modified to include more than one thiol and the glucagons cross link the disulfide side chains of the nanogel.
A method of making glucagon-containing nanogels, comprising the steps of:
- a) producing a copolymer comprising first methacrylate units and second methacrylate units, wherein the first methacrylate units comprise trehalose side chains;
- and the second methacrylate units comprise disulfide side chains;
- b) reacting glucagon with a thiolating agent to produce modified glucagon having more than one thiol;
- and
- c) conjugating the modified glucagon into the co-polymer to form glucagon-containing nanogels.
Dependencies are inferred from the claim text.