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US 11,951,168 B2Granted
Composition containing buffer
AssigneePerseus Proteomics Inc.
InventorsYuichi Funase, Masahiro Kurokawa
Life of this patent
Estimating the term…
Abstract
Provided is a composition containing a buffer to be used at the time of labeling of a chelated targeting agent with 90Y, 153Sm, 165Dy, 165Er, 166Ho, or 177Lu. At least one kind of buffer selected from the group consisting of benzoic acid, maleic acid, fumaric acid, succinic acid, and salts thereof is incorporated in a composition containing a chelated targeting agent.
Claims8
A method of producing a radioactive metal-labeled chelated targeting agent, the method comprising:
- adding a radioactive metal selected from the group consisting of 90Y, 153Sm, 165Dy, 165Er, 166Ho, and 177Lu to a mixture of a chelated targeting agent selected from the group consisting of a low-molecular-weight compound, a peptide, a protein, an antibody, an antibody fragment, and a nucleic acid chelated with at least one member selected from the group consisting of
- and at least one buffer selected from the group consisting of benzoic acid, maleic acid, and salts thereof to form a complex between the radioactive metal and the chelated targeting agent,
- wherein a pH of a reaction liquid at the time of labelling of the chelated targeting agent with the radioactive metal is adjusted to fall within a range of the pKa value of the buffer plus or minus 1.5.
A method of producing a radioactive metal-labeled chelated targeting agent, the method comprising:
- adding a radioactive metal selected from the group consisting of 90Y, 153Sm, 165Dy, 165Er, 166Ho, and 177Lu to a mixture of a chelated targeting agent selected from the group consisting of a low-molecular-weight compound, a peptide, a protein, an antibody, an antibody fragment, and a nucleic acid chelated with at least one member selected from the group consisting of:
- and at least one compound selected from the group consisting of benzoic acid, maleic acid, a conjugate base of benzoic acid, a conjugate base of maleic acid, a mixture of benzoic acid and a conjugate base of benzoic acid, and a mixture of maleic acid and a conjugate base of maleic acid, to form a complex between the radioactive metal and the chelated targeting agent,
- wherein a pH of a reaction liquid at the time of labelling of the chelated targeting agent with the radioactive metal is adjusted to fall within a range of the pKa value of the compound plus or minus 1.5.
Dependencies are inferred from the claim text.