Conjugated vaccine carrier proteins
AssigneeVaxcyte, Inc.
InventorsJeffery C. Fairman, Jon H. Heinrichs, Wei Chan
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Abstract
Methods for the production of immunogenic compositions containing a non-natural amino acid are disclosed. The non-natural amino acid can be a site for attachment of antigens, such as bacterial capsular polysaccharides, to make immunogenic conjugates. Bio-orthogonal attachment chemistry incorporated into the non-natural amino acids allows for more efficient and potent antigen presentation to the immune system, simplified purification, and more well-defined structure of these semi-synthetic immunogens.
Claims4
A carrier protein for use in a crosslinked protein-antigen conjugate vaccine comprising at least 98% sequence identity to SEQ ID NO:9, wherein:
- a 2-amino-3-(4-(azidomethyl)phenyl)propanoic acid (pAMF) non-natural amino acid (nnAA) is present at residues 34, 213, 245, 265, 386, and 527 numbered according to SEQ ID NO:9.
A carrier protein for use in a crosslinked protein-antigen conjugate vaccine comprising at least 98% sequence identity to SEQ ID NO:14, wherein:
- a 2-amino-3-(4-(azidomethyl)phenyl)propanoic acid (pAMF) non-natural amino acid (nnAA) is present at residues 34, 213, 245, 265, 386, and 527 numbered according to SEQ ID NO:14.
Dependencies are inferred from the claim text.