Streptococcus pneumoniae capsular polysaccharides and immunogenic conjugate thereof

An immunogenic composition comprising 24 different Streptococcus pneumoniae capsular polysaccharide-protein conjugates,

• wherein each capsular polysaccharide-protein conjugate comprises a carrier protein conjugated to a capsular polysaccharide derived from a different serotype of Streptococcus pneumoniae, wherein the Streptococcus pneumoniae serotypes are selected from serotypes 1, 2, 3, 4, 5, 6A, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F, and 33F;
• and
• wherein the capsular polysaccharides from serotypes 1 and 5 are conjugated to tetanus toxoid and the capsular polysaccharides from serotypes 2, 3, 4, 6A, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19A, 19F, 20, 22F, 23F, and 33F are conjugated to CRM197,
• wherein the capsular polysaccharide from serotype 2 has a molecular weight of 100 to 400 kDa and a degree of oxidation of 2 to 18,
• wherein the capsular polysaccharide from serotype 9N has a molecular weight of 200 to 700 kDa and a degree of oxidation of 2 to 19,
• wherein the capsular polysaccharide from serotype 17F has a molecular weight of 400 to 900 kDa and a degree of oxidation of 1 to 22, and
• wherein the capsular polysaccharide from serotype 20 has a molecular weight of 400 to 800 kDa and a degree of oxidation of 4 to 16.