Pea-derived peptide with muscle-building effect and preparation method thereof, and drug and use

A method for preparing a pea-derived peptide comprising one or more of the following polypeptides as separate peptide fragments:

• PP1 with an amino acid sequence shown in SEQ ID NO:
• 1, PP2 with an amino acid sequence shown in SEQ ID NO:
• 2, PP3 with an amino acid sequence shown in SEQ ID NO:
• 3, PP4 with an amino acid sequence shown in SEQ ID NO:
• 4, and PP5 with an amino acid sequence shown in SEQ ID NO:
• 5, wherein the method comprises the following steps:
• 1) conducting enzymatic hydrolysis on a pea protein under an action of an alkaline protease to obtain a first enzymatic hydrolysate;
• 2) conducting enzymatic hydrolysis on the first enzymatic hydrolysate under an action of papain and cellulase to obtain a second enzymatic hydrolysate;
• 3) inactivating the second enzymatic hydrolysate, then filtering inactivated second enzymatic hydrolysate through a ceramic membrane, filtering through an organic membrane, and collecting a permeate to obtain a pea-derived peptide;
• 4) subjecting the pea-derived peptide to gel chromatography, and collecting a fraction S2 12-18 min;
• 5) subjecting the fraction S2 to separation and purification by preparative liquid chromatography, and collecting a fraction W5 at 55-63 min;
• and
• 6) subjecting the fraction W5 to separation and purification by analytical liquid chromatography, and sequentially collecting 5 polypeptide fragments at a PP1 time interval, a PP2 time interval, a PP3 time interval, a PP4 time interval, and a PP5 time interval, respectively, to obtain PP1, PP2, PP3, PP4, and PP5.