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AssigneesNorth Carolina State University, The Trustees of the University of Pennsylvania
InventorsJessica M. Gilbertie, Lauren V. Schnabel, Thomas P. Schaer
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An acellular, anion-depleted platelet-derived peptide-rich composition comprising proteins, polypeptides and peptides <10 kDa in size, wherein the composition has anti-microbial and/or anti-inflammatory activity is disclosed. The composition can be substantially free of non-active and immunogenic factors. The composition can have a platelet-to-bacteria ratio ≥1000:1. The composition can comprise plasma at a range of ≥10% to ≤50% plasma, optionally about 10% plasma. Methods of using the composition are also disclosed. Also disclosed is a method for preparing an acellular platelet-derived peptide-rich composition having anti-microbial and/or anti-inflammatory activity, the method comprising: providing a starting material comprising platelet-rich plasma processed to contain ≥1,000,000 platelet/μL, ≤100 leukocytes/μL and ≤10 erythrocyte/μL in plasma; lysing and/or activating the starting material; contacting the starting material with an ion exchange matrix and/or a size exchange matrix; and isolating the acellular platelet-derived peptide-rich composition.
An acellular cationic platelet lysate composition derived from platelet-rich plasma processed to contain ≥1,000,000 platelet/μL, ≤100 leukocytes/μL and 10 erythrocyte/μL, wherein platelets are lysed in plasma having proteolytic activity and cellular debris is removed, wherein the composition comprises cationic proteins, polypeptides and peptides <10 kDa in size, is depleted of anionic proteins, polypeptides and peptides >10 kDa in size, and comprises ≥10% plasma.
Dependencies are inferred from the claim text.