Method for preventing the formation of calcified deposits and for inactivating xenoantigens in biological matrices
AssigneeBiocompatibility Innovation S.R.L.
InventorsFilippo Naso, Alessandro Gandaglia
Life of this patent
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Abstract
The present invention discloses a method for preventing the formation of calcified deposits or inside an isolated biological matrix comprising the step of contacting said isolated biological matrix with a solution comprising a mixture of phenolic compounds.
Claims17
A method for preventing the formation of calcified deposits on or inside an isolated biological matrix after said isolated biological matrix is implanted in a body, wherein the method comprises the step of:
- contacting a biological matrix with a solution comprising an effective amount of a mixture of phenolic compounds to obtain said isolated biological matrix;
- and
- implanting in the body said isolated biological matrix, wherein said isolated biological matrix comprises no calcified deposits on or inside said isolated biological matrix after said isolated biological matrix is implanted in the body for a period of at least 2 months.
A method for preventing the formation of calcified deposits on or inside an isolated biological matrix, the method comprising a step of contacting for a period of less than two hours at the temperature of 35±2° C. in the dark a biological matrix with a solution comprising the following effective combinations of phenylpropanoids (Compound 1 and Compound 2) to obtain said isolated biological matrix:
- Compound 1 - quantity
- Compound 2 - quantity
- (mg/ml) ± 0.5 mg/ml
- (mg/ml) ± 0.5 mg/ml
- Resveratrol 3 mg/ml
- Aloin 1.5 mg/ml
- Resveratrol 3 mg/ml
- Cynarin 2 mg/ml
- Epigallocatechin 2 mg/ml
- Aloin 1.5 mg/ml
- Tannic Acid 4 mg/ml
- Chlorogenic Acid 4 mg/ml
- Tannic Acid 4 mg/ml
- Caffeic Acid 2 mg/ml
- Chlorogenic Acid 4 mg/ml
- Hydroxytyrosol 4 mg/ml
- Rosmarinic Acid 2.5 mg/ml
- Aloin 1.5 mg/ml
- Naringenin 1 mg/ml
- Gallic Acid 1.5 mg/ml
- Hesperetin 2 mg/ml
- Gallic Acid 1.5 mg/ml;
- and
- implanting in a body said isolated biological matrix, wherein said isolated biological matrix comprises no calcified deposits on or inside said isolated biological matrix after implantation of said isolated biological matrix in the body for a period of at least 2 months.
Dependencies are inferred from the claim text.