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US 11,946,896 B1Granted
Electrochemical micro-sensor for GABA detection
AssigneeLouisiana Tech Research Corporation
InventorPrabhu Arumugam
Life of this patent
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Abstract
A GABA detecting probe having a probe body with both a glutamate (Glu) micro-sensor and a GABA micro-sensor positioned on the probe body. The Glu micro-sensor and the GABA micro-sensor include electrodes having a surface modification with (i) GOx and a binding matrix, and (ii) GABASE, GOx, and the binding matrix, respectively. The sensors are positioned no further apart than 250 um and includes a sentinel site located on the probe body.
Claims20
A GABA detecting probe comprising:
- (a) a probe body;
- (b) a Glu micro-sensor located on the probe body, the Glu micro-sensor including at least one Pt electrode having a surface modification with GOx and a binding matrix;
- (c) a GABA micro-sensor located on the probe body, the GABA micro-sensor including at least one Pt electrode having a surface modification with GABASE, GOx, and the binding matrix, the GABA and Glu micro-sensors being positioned no further than 250 um apart on the probe body;
- (d) a sentinel site located on the probe body, the sentinel site including at least one Pt electrode having a surface modification with the binding matrix, but no GOx or GABASE;
- and
- (e) wherein the Pt electrodes each include (i) a surface area of less than 35,000 um2, and (ii) a connecting lead attached thereto and extending along the probe body.
3 more elements
A method of detecting GABA in a brain cell mass comprising the steps of:
- (a) inserting a probe body into the brain cell mass, the probe comprising:
- (i) a Glu micro-sensor located on the probe body, the Glu micro-sensor including at least one Pt electrode having a surface modification with GOx and a binding matrix;
- (ii) a GABA micro-sensor located on the probe body, the GABA micro-sensor including at least one Pt electrode having a surface modification with GABASE, GOx, and the binding matrix, the GABA and Glu micro-sensors being positioned no further than 250 um apart on the probe body;
- (iii) a sentinel site located on the probe body, the sentinel site including at least one Pt electrode having the binding matrix but no GOx or GABASE;
- and
- (iv) wherein the Pt electrodes each include (1) a surface area of less than 35,000 um2, and (2) a connecting lead attached thereto and extending along the probe body;
- (b) receiving at a potentiostat electrical signals from the connecting leads and determining an electro-potential difference between the GABA micro-sensor and Glu micro-sensor.
4 more elements
A method of manufacturing a GABA probe comprising the steps of:
- (a) providing a probe body;
- (b) forming a Glu micro-sensor on the probe body, the Glu micro-sensor including at least one Pt electrode having a surface modification with GOx and a binding matrix;
- (c) forming a GABA micro-sensor on the probe body, the GABA micro-sensor including at least one Pt electrode having a surface modification with GABASE, GOx, and the binding matrix, the GABA and Glu micro-sensors being positioned no further than 250 um apart on the probe body;
- (d) forming a sentinel site on the probe body, the sentinel site including at least one Pt electrode having the binding matrix but no GOx or GABASE;
- and
- (e) wherein the Pt electrodes each include (i) a surface area of less than 35,000 um2, and (ii) a connecting lead is attached thereto and extends along the probe body.
3 more elements
Dependencies are inferred from the claim text.