Back to results
US 11,946,083 B2Granted
Method for the microbial production of specific natural capsaicinoids
AssigneeConagen Inc.
InventorsHui Chen, Xiaodan Yu, LanLan Zhou, Hongxue Wang, Min Wang
Life of this patent
Estimating the term…
Abstract
The present invention relates to the production of capsaicinoid compounds including Capsaicin and Nonivamide via microbial fermentation.
Claims20
A biosynthetic method of making a glucoside of a capsaicinoid of interest comprising:
- a) expressing an acyl-CoA synthetase (ACS) and capsaicin synthase (CS) in a transformed cellular system;
- b) expressing a UDP-glucosyltransferase in the transformed cellular system, wherein the UDP-glucosyltransferase is a CaUGT2 from C. roseus and either is encoded by a DNA sequence at least 75% identical to SEQ ID NO:
- 5 and/or comprises an amino acid sequence at least 90% identical to SEQ ID NO:
- 6;
- c) growing the cellular system in a medium;
- and,
- d) producing the glucoside of a capsaicinoid of interest.
4 more elements
Dependencies are inferred from the claim text.