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US 11,946,075 B2Granted
Freeze dried viral nanoparticle constructs
AssigneeCase Western Reserve University
InventorsJonathan Pokorski, Nicole Steinmetz, Parker Lee
Life of this patent
Estimating the term…
Abstract
A method of producing plant virus-like particles includes freeze drying an aqueous solution of plant virus particles to produce a substantially RNA-free plant virus-like particles.
Claims19
A method of producing plant virus-like particles, the method comprising:
- freeze drying an aqueous solution of plant virus particles to produce substantially RNA-free plant virus-like particles, wherein the plant virus particles comprise a plant virus from the family Tymoviridae.
A method of producing empty plant virus-like particles, the method comprising:
- freezing an aqueous solution of plant virus particles at a temperature and for an amount of time that allows the plant virus particles to eject the encapsulated genomic RNA while maintaining an intact capsid conformation, wherein the plant virus particles comprise a plant virus from the family Tymoviridae;
- and
- sublimating the frozen aqueous solution of plant virus particles to remove water and RNA in the plant virus capsid.
Dependencies are inferred from the claim text.