Genetically modified probiotics for oral delivery of renin-angiotensin related therapeutic proteins and peptides
AssigneeUniversity of Florida Research Foundation, Inc.
InventorsQiuhong Li, Mohan Raizada
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Abstract
Provided herein are polynucleic acids and expression vectors for the expression and secretion of angiotensin peptide fragments (e.g., angiotensin-(1-7)) in probiotic bacteria. Provided herein are also probiotic compositions that enable efficient, cost-effective and patient friendly oral therapeutics for treating diverse pathological conditions that involve the renin-angiotensin system (RAS), e.g., pulmonary hypertension, diabetes, diabetic complications, cardiovascular diseases, and ocular inflammatory and neurodegenerative diseases.
Claims21
An expression vector comprising a polynucleic acid for expressing ACE2 in a bacterium, the polynucleic acid comprising:
- a promoter,
- a nucleic acid encoding a secretion signal peptide,
- a nucleic acid encoding a carrier protein, wherein the nucleic acid encoding the carrier protein has:
- (i) a polynucleic acid sequence having at least 80% sequence identity to SEQ ID NO:
- 3 or
- (ii) a polynucleic acid sequence having at least 95% sequence identity to SEQ ID NO:
- 18,
- a nucleic acid encoding ACE2, and;
- wherein the nucleic acids encoding the secretion signal peptide, carrier protein and ACE2 encode a fusion protein and are operably linked to the promoter.
Dependencies are inferred from the claim text.