Tetravalent dengue vaccine
AssigneeInternational Centre for Genetic Engineering and Biotechnology
InventorsNavin Khanna, Viswanathan Ramasamy
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Abstract
The invention provides a recombinant polypeptide comprising the EDIII domain of each of Dengue virus serotype DENV-1, DENV-2, DENV-3, and DENV-4 linked to the N-terminal of HBsAg.
Claims14
A bio-nanoparticle comprising a recombinant tetravalent domain (EDIII-T) polypeptide comprising an EDIII domain of each of Dengue virus serotypes DENY-1, DENV-2, DENV-3, and DENV-4, wherein the C-terminal end of EDIII-T is fused to the N-terminal end of a surface antigen of Hepatitis B virus (HBsAg) polypeptide.
A method of producing a bio-nanoparticle comprising an EDIII-T polypeptide comprising an EDIII domain of each of Dengue virus serotypes DENY-1, DENV-2, DENV-3, and DENV-4, wherein the C-terminal end of EDIII-T is fused to the N-terminal end of an HBsAg polypeptide, comprising culturing a host cell transformed or transfected with a nucleic acid sequence encoding the EDIII-T polypeptide fused to the HBsAg polypeptide under appropriate conditions and recovering the expressed bio-nanoparticle.
A method for producing dengue virus like particles (VLPs) for preparation of a vaccine comprising:
- (a) introducing an expression vector into cultured cells, wherein the expression vector comprises a nucleic acid encoding an EDIII-T polypeptide comprising an EDIII domain of each of Dengue virus serotypes DENY-1, DENV-2, DENV-3, and DENV-4, wherein the C-terminal end of EDIII-T is fused to the N-terminal end of an HBsAg polypeptide;
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- (b) recovering the dengue VLPs.
A recombinant expression system comprising an expression vector, wherein the expression vector comprises a nucleic acid encoding an EDIII-T polypeptide comprising an EDIII domain of each of Dengue virus serotypes DENY-1, DENV-2, DENV-3, and DENV-4, wherein the C-terminal end of EDIII-T is fused to the N-terminal end of an HBsAg polypeptide, wherein upon expression of the expression vector in a host cell, functional dengue virus like particles are formed comprising each of dengue virus serotypes DENY-1, DENV-2, DENV-3, and DENV-4.
Dependencies are inferred from the claim text.