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US 11,939,616 B2Granted
Compositions and methods for enforcing fucosylation of lactosaminyl glycans in human cells with alpha(1,3)-fucosyltransferases
AssigneeThe Brigham and Women's Hospital, Inc.
InventorRobert Sackstein
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Abstract
The present disclosure provides, inter alia, compositions and methods for enforcing a pattern of cell surface fucosylated lactosaminyl glycans on a human cell. In certain embodiments, the compositions and/or methods utilize one or more members of the α(1,3)-fucosyltransferase family. In certain embodiments, a process for custom-modifying a fucosylated lactosaminyl glycan on a human cell is disclosed.
Claims4
A process for custom engineering a fucosylated lactosaminyl glycan comprising:
- (a) determining a desired enforced glycosylation pattern for a target human cell, wherein the enforced glycosylation pattern is selected from the group consisting of:
- (i) sLex (+)>>Lex(+), with Di-Fuc-sLex (+), and VIM-2 (−);
- and
- (ii) Lex(+), sLex (−), VIM-2 (−), and Di-Fuc-sLex (−);
- (b) selecting an α(1,3)-fucosyltransferase VI capable of producing the desired glycosylation pattern;
- and
- (c) contacting the target human cell with the selected α(1,3)-fucosyltransferase VI.
4 more elements
A method for identifying a human cell via its glycosignature comprising the steps of:
- (a) providing a population of human cells;
- (b) contacting the population of human cells with an α(1,3)-fucosyltransferase VI;
- (c) detecting fucosylated lactosaminyl glycans Lex, sLex, VIM-2, and Di-Fuc-sLex on the population of human cells of step (b);
- and
- (d) identifying the population of human cells detected in step (c) having an enforced fucosylated lactosaminyl glycan pattern selected from the group consisting of:
- (i) sLex (+)>>Lex(+), with Di-Fuc-sLex (+), and VIM-2 (−);
- and
- (ii) Lex(+), sLex (−), VIM-2 (−), and Di-Fuc-sLex (−).
5 more elements
Dependencies are inferred from the claim text.