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US 11,939,586 B2Granted
Means and methods for improved regeneration
AssigneesVib Vzw, Universiteit Gent
InventorsLieven De Veylder, Jefri Heyman
Life of this patent
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Abstract
The present invention relates to the field of plant genetic engineering, particularly the modulation of gene expression in plant cells for improved regeneration competence. The invention discloses means and methods wherein the Ethylene Response Factor (ERF) and Phytochrome A Signal Transduction 1 (PAT1) transcription factor complex activity in plant cells grants increased regeneration.
Claims16
A composition comprising:
- a first chimeric gene construct and a second chimeric gene construct;
- the first chimeric gene construct comprising the following operably linked DNA elements:
- a) a plant expressible promoter,
- b) a DNA region encoding an Ethylene Response Factor (ERF) comprising an SCL/PAT1 interaction motif, and
- c) a 3′ end region comprising transcription termination and polyadenylation signals functioning in cells of a plant, and
- the second chimeric gene construct comprising the following operably linked DNA elements:
- a) a plant expressible promoter,
- b) a DNA region encoding Phytochrome A Signal Transduction 1 (PAT1), and
- c) a 3′ end region comprising transcription termination and polyadenylation signals functioning in cells of a plant
- wherein expression of the first chimeric gene construct and the second chimeric gene construct in a plant leads to the formation of an ERF/PAT1 complex,
- wherein the ERF comprising an SCL/PAT1 interaction motif is ERF115, and
- wherein the plant has increased plant cell regeneration potential without additional hormones as compared to an otherwise identical plant lacking the first chimeric gene construct and the second chimeric gene construct.
9 more elements
A plant comprising:
- a first chimeric gene construct, a second chimeric gene construct, and an ERF/PAT1 complex;
- the first chimeric gene construct comprising the following operably linked DNA elements:
- a) a plant expressible promoter,
- b) a DNA region encoding an Ethylene Response Factor (ERF) comprising an SCL/PAT1 interaction motif, and
- c) a 3′ end region comprising transcription termination and polyadenylation signals functioning in cells of the plant, and
- the second chimeric gene construct comprising the following operably linked DNA elements:
- a) a plant expressible promoter,
- b) a DNA region encoding Phytochrome A Signal Transduction 1 (PAT1), and
- c) a 3′ end region comprising transcription termination and polyadenylation signals functioning in cells of the plant
- wherein the ERF comprising an SCL/PAT1 interaction motif is ERF115, and
- wherein the plant has increased plant cell regeneration potential without additional hormones as compared to an otherwise identical plant lacking the first chimeric gene construct and the second chimeric gene construct.
8 more elements
Dependencies are inferred from the claim text.