Method of producing autotrophic organisms with altered photorespiration and improved CO2 fixation
AssigneeMax-Planck-Gesellschaft zur Forderung der Wissenschaften e.V.
InventorsLennart Schada Von Borzyskowski, Tobias Jürgen Erb, Jan Zarzycki
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Abstract
The present invention relates to autotrophic microorganisms with altered photorespiration and improved CO2 fixation as well as a method of producing said autotrophic microorganisms. Particularly, the autotrophic microorganisms show an improved growth rate, productivity and energy conversion efficiency.
Claims16
A method for the production of autotrophic microorganisms with altered photorespiration comprising introducing into an autotrophic microorganism expressing (i) phosphoglycolate phosphatase activity one or more nucleic acids encoding polypeptides having the enzymatic activities of:
- (ii) glyoxylate reductase,
- (iii) erythro-β-hydroxyaspartate aldolase belonging to the EC class 4.1.3.14,
- (iv) erythro-β-hydroxyaspartate dehydratase belonging to the EC class 4.3.1.20,
- (v) iminosuccinate reductase and
- (vi) aspartate-glyoxylate transaminase, wherein the introduction of the one or more nucleic acids results in an altered autotrophic microorgansim having de novo expression of polypeptides having the enzymatic activities of
- (ii) glyoxylate reductase,
- (iii) erythro-β-hydroxyaspartate aldolase belonging to the EC class 4.1.3.14,
- (iv) erythro-β-hydroxyaspartate dehydratase belonging to the EC class 4.3.1.20,
- (v) iminosuccinate reductase, and
- (vi) aspartate-glyoxylate transaminase.
An autotrophic microorganism expressing (i) phosphoglycolate phosphatase activity and comprising one or more heterologous nucleic acids encoding polypeptides having the enzymatic activities of (ii) glyoxylate reductase, (iii) erythro-β-hydroxyaspartate aldolase belonging to the EC class 4.1.3.14, (iv) erythro-β-hydroxyaspartate dehydratase belonging to the EC class 4.3.1.20, (v) iminosuccinate reductase and (vi) aspartate-glyoxylate transaminase.
Dependencies are inferred from the claim text.