Construct of self-circularization RNA

A self-circularization RNA construct comprising, from the 5′-terminus to the 3′-terminus:

• a) an internal guide sequence (IGS) region comprising guanine (G) at the 5′-end;
• b) a ribozyme region wherein the ribozyme region is a Group I intron ribozyme;
• c) a gene of interest (GOI) region;
• and
• d) a fragment containing target splice site comprising uracil (U) at the 3′-end,
• wherein the 5′-end G of the IGS region and the 3′-end U of the target splice site-containing fragment form a G :
• U wobble base pair,
• wherein the a) IGS region comprises a sequence of 2 to 10 contiguous nucleotides, which is reverse complementary to a 3′-end portion nucleotide sequence of the d) target splice site-containing fragment, and
• wherein the self-circularization RNA construct allows production of a circular RNA comprising the c) gene of interest region and the d) target splice site-containing fragment, but not the b) ribozyme region and the a) IGS region.