Back to results
US 11,939,557 B2Granted
Beta-glucosidase expressing yeast for enhanced flavor and aroma in beverage production
AssigneeDanstar Ferment AG
InventorsCharles F. Rice, Aaron Argyros
Life of this patent
Estimating the term…
Abstract
The present disclosure concerns recombinant yeast host cells expressing heterologous enzymes for hydrolyzing flavor compounds glycosidically bound to a sugar molecule. The recombinant yeast host cells can be used in a subsequent production process to make alcoholic beverage products such as wines and beers.
Claims20
A recombinant yeast host cell for improving a flavor profile of a fermented beverage by hydrolyzing a non-volatile conjugate, wherein the fermented beverage is obtained by fermentation of a fermentable medium with the recombinant yeast host cell, and the non-volatile conjugate is of formula (I):
- VFC—SM (I)
- where:
- VFC is a flavor compound that is volatile when released from the non-volatile conjugate,
- SM is a sugar molecule, and
- “—” is a β-glycosidic linkage covalently attaching the VFC to the SM, and capable of being hydrolyzed;
- wherein the recombinant yeast host cell:
- has a heterologous nucleic acid molecule encoding one or more heterologous polypeptide having 1,4-β-glucosidase activity for hydrolyzing the β-glycosidic linkage to release the VFC from the non-volatile conjugate;
- has a native ethanol production pathway;
- and
- is a brewing or wine strain.
7 more elements
Dependencies are inferred from the claim text.