Protein composition and methods for analysing microbiota
AssigneeUniversity of Ottawa
InventorsJoseph Michel Daniel Figeys, Alain Christophe Stintzi, David R. Mack, Xu Zhang, Zhibin Ning
Life of this patent
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Abstract
A method of isotope-labelling a microbiota sample. It involves providing a first microbiota sample that was obtained from a given source; exposing the first microbiota sample to an isotope enriched medium; and culturing the exposed first microbiota sample in the isotope enriched medium to obtain an isotope-labelled microbiota sample, wherein the isotope labelled metaproteome of the isotope-labelled microbiota sample is taxon specific for taxa present in the first microbiota sample when initially obtained from the given source.
Claims13
A method of high throughput screening of multiple microbiota samples from a human subject, soil, or animal for metaproteomic analysis of said samples comprising:
- providing proteome extracts from a plurality of microbiota samples from one or more human subjects, soil, or animals in culture;
- spiking said proteome extracts with an isotope-labelled standard, which comprises a proteome of microbiota from a given human, soil, or animal microbiota sample labelled with an isotope by culturing said microbiota from a given human, soil, or animal microbiota in an isotope enriched medium;
- performing a pre-screening using a metaproteomic analysis to identify changes in microbiomes of said plurality of microbiota samples;
- selecting said microbiomes exhibiting changes;
- and
- analyzing the selected microbiomes to characterize the changes.
Dependencies are inferred from the claim text.