Biosynthesis of curcuminoids in mammalian cells
AssigneeUniversity of Kentucky Research Foundation
InventorsLogan W. Warriner, Daniel W. Pack
Life of this patent
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Abstract
A method of making curcuminoids in a mammalian cell. The method of making a curcuminoid in a mammalian cell includes expressing one or more enzymes in the mammalian cell, the enzymes being selected from the group consisting of tyrosine ammonia lyase (TAL), 4-coumaroyl-CoA ligase (4CL1), curcuminoid synthase (CUS), diketide-CoA synthase (DCS), curcumin synthase (CURS1), 4-coumarate 3-hydroxylase (C3H), caffeoyl-CoA 3-O-methyltransferase (CCoAMT), and acetyl-CoA carboxylase (ACC). The expressing of the one or more enzymes converts a starting material, such as tyrosine or ferulic acid, to the curcuminoid. Also provided herein are therapeutic uses for the curcuminoid made in a mammalian cell.
Claims19
A method of making a curcuminoid in a mammalian cell, the method comprising:
- expressing enzymes 4-coumaroyl-CoA ligase (4CL1) and one of 1) curcuminoid synthase (CUS) and 2) curcumin synthase (CURS1) and diketide-CoA synthase (DCS) in the mammalian cell, and optionally further expressing in the mammalian cell one or more additional enzymes selected from the group consisting of tyrosine ammonia lyase (TAL), DCS, CURS1, 4 coumarate 3- hydroxylase (C3H), caffeoyl-CoA 3-O-methyltransferase (CCoAOMT), and acetyl-CoA carboxylase (ACC);
- and
- contacting the mammalian cell expressing the enzymes with a starting material being tyrosine, ferulic acid or p-coumaric acid to convert the starting material to the curcuminoid.
A method of making a curcuminoid in a mammalian cell, the method comprising:
- introducing ferulic acid to the mammalian cell, and performing one of the following:
- (a) expressing 4-coumaroyl-CoA ligase (4CL1) and curcuminoid synthase (CUS) in the mammalian cell;
- (b) expressing tyrosine ammonia lyase (TAL), 4CL1, and CUS in the mammalian cell;
- (c) expressing TAL, 4CL1, CUS, 4-coumarate 3-hydroxylase (C3H), and caffeoyl-CoA 3-0-methyltransferase (CCoAOMT) in the mammalian cell;
- or
- (d) expressing TAL, 4CL1, C3H, CCoAOMT, curcumin synthase (CURS 1), and diketide-CoA synthase (DCS) in the mammalian cell;
- wherein the expressing the enzymes converts ferulic acid to the curcuminoid.
Dependencies are inferred from the claim text.