Metabolically competent cells, methods of making, and uses thereof
AssigneesUniversity of Florida Research Foundation, Inc., The Research Foundation for The State University of New York
InventorsChristopher Dillon Vulpe, Amin Sobh, David Michael Faulkner, Abderrahmane Tagmount, Michael Fasullo
Life of this patent
Estimating the term…
Abstract
Provided herein are genetically engineered cells containing one or more modulated metabolic genes, where the expression of the modulated metabolic gene(s) can be greater than that of an unmodified control. Also provided herein are methods of making the genetically engineered cells using synergistic activation mediator CRISPR-Cas9. Further provided herein are high throughput assays that can employ the genetically engineered cells provided herein.
Claims2
A genetically engineered cell line comprising an immortalized cell line comprising:
- i) one or more modulated metabolic genes, wherein the expression of the one or more modulated metabolic genes is greater than the expression in an unmodified control cell,
- wherein the one or more modulated metabolic genes are selected from the group consisting of:
- CYP1A1, CYP1A2, CYP2B6, CYP2E1, and CYP3A4;
- ii) one or more sgRNAs for one or more metabolic genes, wherein the one or more sgRNAs are one or more of SEQ ID NOs:
- 1-15 and 19-24;
- and
- iii) an inactive Cas9 (dCas9) operatively linked to a VP64 transcriptional activator.
Dependencies are inferred from the claim text.