US 11,932,861 B2 · Virus-based replicon for plant genome editing without inserting replicon into plant genome and uses thereof

Virus-based replicon for plant genome editing without inserting replicon into plant genome and uses thereof

AssigneeIndustry-Academic Cooperation Foundation Gyeongsang National University

InventorsJae Yean Kim, Tien Van Vu, Jihae Kim, Se Jeong Jeong, Hyun Jeong Kim, Seo-Jin Park, Mil Thi Tran, Velu Sivankalyani, Yeon Woo Sung, Thi Hai Duong Doan, Dibyajyoti Pramanik, Mahadev Rahul Shelake, Geon Hui Son

Life of this patent

Estimating the term…

Abstract

A recombinant vector according to an embodiment is for genome editing without inserting a replicon into the plant genome in a T0 generation plant. The recombinant vector includes a geminivirus-based replicon between the sequence of LB (left border) and sequence of RB (right border) of Ti plasmid. A method of genome editing without inserting a replicon into the plant genome in a T0 generation plant according to an embodiment includes transforming a plant cell by inserting a foreign gene to the aforementioned recombinant vector.

Claims4

Dependent claims

A recombinant vector for genome editing without inserting a replicon into plant genome in a T0 generation plant, the recombinant vector including a geminivirus-based replicon between the sequence of LB (left border) and sequence of RB (right border) of Ti plasmid,

wherein the geminivirus is BeYDV (Bean Yellow Dwarf Virus),
wherein the replicon has a first long intergenic region (LIR), a Rep/RepA protein-coding sequence, a short intergenic region (SIR), a CaMV 35S terminator, a Cpf1 coding sequence, a CaMV 35s promoter having AtUBQ intron, U6-double gRNAs, a first template DNA site for gene-editing, a NOS promoter, NPTII coding sequence, a OCS (octopine synthase) terminator, a CaMV 35S promoter, a second template DNA site for gene-editing, and a second long intergenic region (LIR) that are sequentially connected.

Dependencies are inferred from the claim text.