Method for producing alkaline phosphatase, alkaline phosphatase obtained using said method, and vector and transformant for production thereof

A vector for Aspergillus transformation, comprising:

• (a) an open reading frame encoding an alkaline phosphatase (ALP);
• and
• (b) an Aspergillus strain-derived N-terminal secretory signal peptide,
• wherein the ALP comprises 90% or greater homology with either of the amino acid sequences set forth in SEQ ID NOs:
• 31 or 32 and has an amino acid substitution at position 122, 249, 251 and/or 410, wherein the Asn at position 122 is a substitution to Gln or Lys, the Asn at position 249 is a substitution to Gln, Asp or His, the Thr at position 251 is a substitution to Cys, and the Asn at position 410 is a substitution to Gln or Lys,
• wherein the Aspergillus strain-derived N-terminal secretory signal peptide is 5′ to the open reading frame encoding the ALP and comprises 90% or greater homology with any one of the sequences set forth in SEQ ID NOs:
• 2 to 15, and
• wherein the ALP has decreased protein glycosylation levels compared to a wild-type ALP.