Chloramphenicol resistant split protein and uses thereof

A construct system comprising:

• (i) a first nucleic acid construct comprising a first polynucleotide having a nucleic acid sequence that encodes a N-terminal fragment of chloramphenicol acetyl transferase (CAT), said N-terminal fragment comprising a first portion of the catalytic active site of said CAT, said N-terminal fragment being devoid of acetylating activity;
• and
• (ii) a second nucleic acid construct comprising a second polynucleotide having a nucleic acid sequence that encodes a C terminal fragment of said CAT, said C-terminal fragment comprising a second portion of the catalytic active site of said CAT, said C-terminal fragment being devoid of acetylating activity;
• and
• wherein said N-terminal fragment is capable of associating with said C-terminal fragment to generate an active CAT that is capable of acetylating chloramphenicol,
• wherein said N terminal fragment consists of the amino acid sequence as set forth in SEQ ID NO:
• 2 or 6;
• and/or
• said C-terminal fragment consists of the amino acid sequence as set forth in SEQ ID NOs:
• 3 or 7.