Feed composition supplemented with a protease combination

A method of improving digestibility of proteins in animal feed, comprising:

• adding to the animal feed i) a protease combination having neutral metalloprotease and serine alkaline protease activity and ii) amylase, wherein the protease combination or the protease mixture has a ratio of total activity of neutral metalloprotease:serine alkaline protease of from 4:1 to 1.5:1,
• the amylase is at least one selected from the group consisting of an α-amylase, a β-amylase, and a γ-amylase;
• the protease combination or the protease mixture and amylase have a ratio of total activity of protease:amylase of from 20:1.13 to 1:5;
• and
• the protease combination or the protease mixture is derived from Bacillus amyloliquefaciens.

A method of optimizing nutritional value of an animal diet or feed, comprising:

• adding to the animal diet or feed i) a protease combination having neutral metalloprotease and serine alkaline protease activity and ii) amylase, wherein the protease combination has a ratio of total activity of neutral metalloprotease:serine alkaline protease of from 4:1 to 1.5:1,
• the amylase is at least one selected from the group consisting of an α-amylase, a β-amylase, and a γ-amylase;
• the protease combination or the protease mixture and amylase have a ratio of total activity of protease:amylase of from 20:1.13 to 1:5;
• and
• the protease combination or the protease mixture is derived from Bacillus amyloliquefaciens.

A feed additive or feed supplement, comprising:

• a protease combination or a protease mixture having neutral metalloprotease, serine alkaline protease activity;
• and
• an amylase,
• wherein the neutral metalloprotease and serine alkaline protease have a ratio of total activity of neutral metalloprotease:serine alkaline protease of from 4:1 to 1.5:1;
• the amylase is at least one selected from the group consisting of an α-amylase, a β-amylase, and a γ-amylase;
• the protease combination or the protease mixture and amylase have a ratio of total activity of protease:amylase of from 20:1.13 to 1:5;
• and
• the protease combination or the protease mixture is derived from Bacillus amyloliquefaciens.

An enzyme composition, comprising:

• a protease combination or a protease mixture having neutral metalloprotease and serine alkaline protease activity;
• and
• amylase,
• wherein the neutral metalloprotease and serine alkaline protease have a ratio of total activity of neutral metalloprotease:serine alkaline protease of from 4:1 to 1.5:1;
• the amylase is at least one selected from the group consisting of an α-amylase, a β-amylase, and a γ-amylase;
• the protease combination or the protease mixture and amylase have a ratio of total activity of protease:amylase of from 20:1.13 to 1:5;
• and
• the protease combination or the protease mixture is derived from Bacillus amyloliquefaciens.

A kit for optimizing the nutritional value of an animal diet or animal feed, comprising:

• (a) a protease enzyme combination or a protease enzyme mixture having neutral metalloprotease and serine alkaline protease activity, and an amylase, wherein:
• the protease enzyme combination or the protease enzyme mixture has a ratio of total activity of neutral metalloprotease:serine alkaline protease of from 4:1 to 1.5:1, and
• the protease enzyme combination or the protease enzyme mixture and amylase have a ratio of total activity of neutral metalloprotease and serine alkaline protease:amylase of from 20:1.13 to 1:5;
• and
• (b) instructions to enable supplementation of the animal diet or the animal feed with the enzyme combination,
• the amylase is at least one selected from the group consisting of an α-amylase, a β-amylase, and a γ-amylase;
• the protease combination or the protease mixture and amylase have a ratio of total activity of protease:amylase of from 20:1.13 to 1:5;
• and
• the protease combination or the protease mixture is derived from Bacillus amyloliquefaciens.