Perfusable-type dual proximal tubule cell construct and producing method thereof for applying in vitro artificialrenal tissue model and renal cell therapy

An renal proximal tubule-on-a-chip comprising:

• a vascular tubule construct which is printed with a first bioink comprising a decellularized substance derived from a mammalian kidney tissue and human umbilical vascular endothelial cells (HUVECs) and configured in a tubular shape;
• and
• a proximal tubule construct which is printed with a second bioink comprising the decellularized substance and renal proximal tubular epithelial cells (RPTECs) and configured in a tubular shape,
• wherein the vascular tubule construct and the proximal tubule construct are at least partially printed in parallel with each other and configured so that the outer surfaces thereof are in contact with each other.

A producing method of a proximal tubule cell construct comprising the steps of:

• producing a decellularized substance derived from a mammalian kidney tissue;
• extracting and preparing human umbilical vascular endothelial cells (HUVECs) and renal proximal tubular epithelial cells (RPTECs) from a living kidney tissue;
• producing a first bioink comprising the decellularized material and the HUVECs;
• producing a second bioink comprising the decellularized material and the RPTECs;
• printing a vascular tubule construct using the first bioink;
• and
• printing a proximal tubule construct using the second bioink,
• wherein the printing of the vascular tubule construct and the printing of the proximal tubule construct are performed while the vascular tubule construct and the proximal tubule construct are at least partially in contact with each other.