Two-photon excited fluorescence microscope for diagnosis of Alzheimer's disease (AD) and mild cognitive impairment (MCI), and pulse compressor including therein
AssigneeElectronics and Telecommunications Research Institute
InventorsSang Kyun Lee, Dong Hoon Song, Hong-Seok Seo, Chul Huh
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Abstract
Provided are a pulse compressor and a two-photon excited fluorescence microscope. The microscope includes a light source which generates a laser beam having a pulse, a pulse compressor which compresses the pulse of the laser beam, an objective lens which provides the laser beam to a specimen, and image sensors which receive the laser beam and obtain images of the specimen. The pulse compressor may include a grating plate, a corner cube provided on one side of the grating plate, and a retroreflector provided on the other side of the grating plate.
Claims16
A two-photon excited fluorescence microscope comprising:
- a light source configured to generate a laser beam;
- a pulse compressor configured to compress a pulse of the laser beam;
- an objective lens configured to direct the laser beam to a specimen;
- image sensors configured to receive the laser beam and obtain surface and internal images of the specimen,
- wherein the pulse compressor comprises:
- a grating plate configured to disperse the laser beam;
- a corner cube provided on a first side of the grating plate and configured to reflect the laser beam to the grating plate;
- and
- a retroreflector provided on a second side of the grating plate and configured to re-reflect the laser beam, which has been transmitted through the grating plate, to the corner cube and the grating plate.
A two-photon excited fluorescence microscope comprising:
- a light source configured to generate a laser beam;
- a pulse compressor configured to compress a pulse of the laser beam;
- an objective lens configured to provide the laser beam to a specimen;
- and
- image sensors configured to receive the laser beam and obtain images of the specimen,
- wherein the pulse compressor comprises:
- a grating plate configured to disperse the laser beam;
- a corner cube provided on a first side of the grating plate and configured to reflect the laser beam to the grating plate;
- and
- a retroreflector provided on a second side of the grating plate and configured to re-reflect the laser beam, which has been transmitted through the grating plate, to the corner cube and the grating plate, and
- wherein the grating plate comprises:
- a first diffraction region;
- a second diffraction region spaced apart from the first diffraction region;
- a third diffraction region between the second diffraction region and the first diffraction region;
- and
- a fourth diffraction region between the third diffraction region and the first diffraction region.
A pulse compressor comprising:
- a grating plate configured to disperse a laser beam;
- a corner cube provided on a first side of the grating plate and configured to reflect the laser beam to the grating plate;
- a retroreflector provided on a second side of the grating plate and configured to re-reflect the laser beam, which has been transmitted through the grating plate, to the corner cube and the grating plate;
- and
- a rotator connected to a side wall of the grating plate, the sidewall positioned between the first side of the grating plate and the second side of the grating plate,
- wherein the rotator is configured to rotate the grating plate to adjust a dispersion value of the laser beam.
Dependencies are inferred from the claim text.