US 11,921,045 B2Granted

Holographic three-dimensional multi-spot light stimulation device and method

AssigneeNational University Corporation Kobe University

InventorsOsamu Matoba, Xiangyu Quan, Hiroaki Wake

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Abstract

A holographic three-dimensional multi-spot light stimulation device is provided with: a three-dimensional imaging holographic optical system A which employs fluorescent exciting light to acquire three-dimensional fluorescence distribution information resulting from fluorescent signal light from a plurality of stimulation target objects; and a three-dimensional light stimulation holographic optical system B which employs a light stimulation hologram generated on the basis of the acquired three-dimensional fluorescence distribution information to form a plurality of light spots in space, to impart stimulation simultaneously to the plurality of stimulation target objects. Furthermore, the three-dimensional light stimulation holographic optical system B is provided with a spatial light phase modulating element 22 and a control unit 25, wherein the control unit 25 generates the light stimulation hologram by controlling the spatial light phase modulating element 22 on the basis of the three-dimensional fluorescence distribution information.

Claims18

Dependent claims

A holographic three-dimensional multi-spot light stimulator device comprising:

a three-dimensional imaging holographic optical system configured to employ fluorescent exciting light to acquire three-dimensional fluorescence distribution information as a self-interfering hologram resulting from fluorescent signal light from a plurality of stimulation target objects;
and
a three-dimensional light stimulation holographic optical system configured to employ a light stimulation hologram which is generated on the basis of the acquired three-dimensional fluorescence distribution information to form a plurality of light spots in space, and to impart stimulation simultaneously to the plurality of stimulation target objects;
and
wherein the three-dimensional light stimulation holographic optical system includes a first spatial light modulator and a control unit;
wherein the control unit is configured to identify the positions of the stimulation target objects based on the three-dimensional fluorescence distribution information with the same or substantially the same spatial resolution and three-dimensional observation range as the three-dimensional imaging holographic optical system, and to calculate the light stimulation hologram for forming a plurality of light spots simultaneously at the positions of the stimulation target objects and to generate the light stimulation hologram by controlling the first spatial light modulator.

3 more elements

A holographic three-dimensional multi-spot light stimulator method comprising:

irradiating a plurality of stimulation objects with fluorescence excitation light;
acquiring hologram information of a three-dimensional fluorescence distribution using a self-interfering fluorescence signal light of the stimulation objects;
reconstructing the acquired hologram information of the three-dimensional fluorescence distribution with a computer, thereby facilitating observation of a state of at least one stimulation object;
generating a hologram for light stimulation that simultaneously forms a plurality of light spots at the positions of a plurality of stimulation target objects identified with the same or substantially the same spatial resolution and three-dimensional observation range as the acquired hologram of the three-dimensional fluorescence distribution;
controlling a spatial light modulator with respect to the pattern of the hologram for light stimulation;
spatially forming a plurality of light spots using the hologram for light stimulation and simultaneously applying light stimulation to a plurality of the stimulation target objects;
and
after applying light stimulation to the plurality of the stimulation target objects, reconstructing the acquired hologram information of the three-dimensional fluorescence distribution with a computer, thereby facilitating observation of a state of at least one stimulation object after application of light stimulation.

5 more elements

Dependencies are inferred from the claim text.

Facts

Application: 17/258,849Filed 9 Jul 2019
Priority: JP 2018-1303099 Jul 2018
Published: US 2021/0293714 A123 Sep 2021
Granted: 5 Mar 2024
Term ends: Not estimatedThe source records don’t support an estimate.

Classification37 codes

G01N 21/6458
Spatial resolved fluorescence measurements; Imaging: fluorescence microscopy
C12Q 1/02
Measuring or testing processes involving enzymes, nucleic acids or microorganisms ; Compositions therefor; Processes of preparing such…
G01B 9/021
Interferometers: using holographic techniques
G01N 21/6402
Fluorescence; Phosphorescence: atomic fluorescence; Laser induced fluorescence