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US 11,920,183 B2Granted
Systems and methods for processing optically tagged beads
Assignee10x Genomics, Inc.
InventorsRajiv Bharadwaj, Zachary Bent
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Abstract
The present disclosure provides methods and systems for generating and processing optically tagged beads. A nucleic acid probe comprising a fluorescent tag may be hybridized to a bead to generate an optically tagged bead. The nucleic acid probe hybridized to the optically tagged bead may be detectable by one or more signals and may be substantially unreactive to one or more chemical reactions performed with, or in the presence of, the optically tagged bead.
Claims19
A method for processing a bead, comprising:
- (i) hybridizing the bead and a modified probe to generate an optically tagged bead, wherein the bead comprises a binding sequence, wherein the modified probe hybridizes to the binding sequence, wherein the modified probe comprises a fluorescent tag and a 3′ chain-terminating modification at a 3′ end of the modified probe, and wherein the 3′ chain-terminating modification at the 3′ end of the modified probe renders the modified probe hybridized to the bead at least 60% less reactive in an extension reaction as compared to an equivalent unmodified nucleic acid probe;
- and
- (ii) detecting one or more signals indicative of the fluorescent tag from the optically tagged bead,
- wherein the optically tagged bead comprises:
- i) a molecule comprising the binding sequence hybridized to the modified probe, and ii) a nucleic acid barcode molecule, wherein the molecule is different from the nucleic acid barcode molecule.
2 more elements
Dependencies are inferred from the claim text.