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US 11,920,171 B1Granted
Synthesis and oxidation of methane
AssigneeAuburn University
InventorSteven Mansoorabadi
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Abstract
The present disclosure describes genes and proteins of the coenzyme F430 synthetic pathway. The genes and proteins in the pathway find uses as isolated nucleic acids, transformation vectors, a transformation media, genetically modified cells, methods of modulating methanogenesis, methods of modulating methane oxidation, methods of making a tetrapyrrole compound, methods of oxidizing methane, methods of biogenic methane synthesis is provided, methods of assaying an organism for potential methanogenic or methanotrophic activity, and isolated proteins.
Claims20
A genetically modified cell comprising:
- a first nucleotide sequence encoding a first Cfb polypeptide, and a second nucleotide sequence encoding a second Cfb polypeptide having at least 70% sequence identity to the first Cfb polypeptide, wherein the first and second Cfb polypeptides are both one of a CfbA (sirohydrochlorin cobaltochelatase), CfbB (cobyrinic acid a,c-diamide synthetase), CfbC (nitrogenase iron protein), CfbD (nitrogenase molybdenum-iron protein), and CfbE (UDP-N-acetylmuramoylalanine:D-glutamate ligase) polypeptide.
Dependencies are inferred from the claim text.