Production of carotenoids and apocarotenoids
AssigneeAgency for Science, Technology and Research
InventorsCongqiang Zhang, Xixian Chen, Heng-Phon Too
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Abstract
A method for producing a carotenoid or apocarotenoid is disclosed. The method comprises the step of expressing in a host cell an expression module comprising an expression vector having a coding region encoding at least one optimised carotenoid or apocarotenoid generating enzyme, the coding region being operably linked to a promoter. A host cell comprising an expression vector having a coding region encoding at least one optimised carotenoid or apocarotenoid generating enzyme, the coding region being operably linked to a promoter is also provided together with a kit.
Claims14
A method for producing a-ionone comprising the step of expressing in an Escherichia coli host cell an expression module comprising an expression vector having a coding region encoding optimized a-ionone generating enzymes, the coding region being operably linked to a promoter,
- wherein the optimized a-ionone generating enzymes are LcyE and CCD1 that form an operon having the structure of LcyE-CCD1, and
- wherein the LcyE is obtained from Lactuca sativa and comprises an N-terminal truncation of 50 amino acids (ΔN50-LsLcyE), and
- wherein the CCD1 is fused with an N-terminal fusion partner.
An Escherichia coli host cell comprising an expression module comprising an expression vector having a coding region encoding optimized α-ionone generating enzymes, the coding region being operably linked to a promoter,
- wherein the optimized α-ionone generating enzymes are LcyE and CCD1 that form an operon having the structure obtained from Lactuca sativa and is N-terminally truncated,
- wherein the LcyE comprises a N-terminal truncation of 50 amino acids (ΔN50-LsLcyE), and
- wherein the CCD1 is fused with an N-terminal fusion partner.
A kit for producing an expression module comprising an expression vector having a coding region encoding optimized α-ionone generating enzymes, the coding region being operably linked to a promoter, wherein the optimized α-ionone generating enzymes are ΔN50-LsLcyE and TrxA-Osmanthus fragrans CCD1 for the production of α-ionone.
Dependencies are inferred from the claim text.