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US 11,913,954 B2Granted
Combination treponemal and non-treponemal syphilis test
AssigneeBio-Rad Laboratories, Inc.
InventorsRavi Kaul, Weiming Zheng, Roger Walker
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Abstract
Provided herein are multiplex assays for detecting antibodies indicative of presence and stage of syphilis infection in an individual. Individuals infected with syphilis produce antibodies directed to syphilis components and the lipid cellular debris associated with the infection. The present disclosure represents the first combination of these diverse antibody targets in a single assay.
Claims15
A method of detecting the presence of syphilis antibodies in a sample comprising:
- (a) contacting the sample with (i) a cardiolipin-lecithin-cholesterol antigen non-covalently attached to a solid support, wherein the cardiolipin-lecithin-cholesterol antigen is unmodified to reduce hydrophobicity;
- and (ii) a Treponema pallidum antigen attached to a solid support;
- (b) washing the solid supports of (i) and (ii) to remove unbound sample;
- (c) contacting the solid supports of (i) and (ii) with a labeled, anti-human immunoglobulin (Ig) antibody;
- and
- (d) detecting the presence of syphilis antibodies by detecting the label on the anti- human Ig antibody.
3 more elements
Dependencies are inferred from the claim text.