Bond-selective intensity diffraction tomography and uses thereof
AssigneeTrustees of Boston University
InventorsJi-Xin Cheng, JIan Zhao, Lei Tian, Alex Matlock
Life of this patent
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Abstract
An example microscope includes a pump laser for providing a first illumination to a sample. A laser array provides a second illumination to the sample. The laser array may include a plurality of laser elements, each providing oblique illuminations to the sample. An illumination collecting source collects the first illumination and the second illumination from the sample. The illumination collecting source may capture transient 3D refractive index (RI) variations in the sample due to the first illumination and second illumination.
Claims26
A microscope comprising:
- a pump laser for providing a first illumination to a sample;
- a laser array for providing a plurality of second illuminations to the sample, wherein the laser array comprises a plurality of laser elements, each providing oblique illuminations to the sample;
- and
- an illumination collection source for collecting scattered illuminations from the sample, the illumination collection source producing intensity images encoding a 3D volume of the sample for each of the oblique illuminations, wherein the illumination collection source captures transient 3D refractive index (RI) variations in the sample due to the illuminations provided by the laser array using the intensity images.
A method for forming a microscope comprising:
- providing, using a pump laser, a first illumination to a sample;
- providing, using a laser array, a plurality of second illuminations to the sample, wherein the laser array comprises a plurality of laser elements, each providing oblique illuminations to the sample;
- and
- collecting, using an illumination collection source, transmitted illuminations from the sample;
- producing, using the illumination collection source, intensity images encoding a 3D volume of the sample for each of the oblique illuminations;
- capturing, using the intensity images, transient 3D refractive index (RI) variations in the sample due to the illuminations provided by the laser array.
A method for performing chemical imaging, the method comprising:
- synchronizing a plurality of probe lasers, a pump laser, and a camera at an acquisition speed;
- applying illuminations from the synchronized probe lasers and pump laser to a sample;
- for each probe laser illumination, collecting a pair of raw images from the sample;
- reconstructing, using an inverse scattering model and the pair of raw images, a pair of 3D refractive index (RI) volume maps;
- and
- performing a subtraction operation on the reconstruction pair of 3D RI volume maps to reveal 3D RI fluctuations of the sample.
Dependencies are inferred from the claim text.