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US 11,913,138 B2Granted
Generating recombinant affinity reagents with arrayed targets
AssigneesArizona Board of Regents on behalf of Arizona State University, The Board of Trustees of the University of Illinois
InventorsJoshua Labaer, Kevin Gorman, Brian Kay, Jie Wang, Ji Qiu
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Abstract
Methods for screening of affinity reagents for many target proteins of interest simultaneously. Arrayed targets (e.g., peptide, protein, RNA, cell, etc.) are used in affinity selection experiments to reduce the amount of target needed and to improve the throughput of discovering recombinant affinity reagents to a large collection of targets.
Claims6
A method for affinity selection with arrayed target material, comprising the steps of:
- a) providing an array comprising arrayed target proteins, wherein the arrayed target proteins comprise MAP2K5, CTBP1, SARA1A, CDK2 and RPS6KA3;
- b) incubating a ribosome display material with the array,
- c) washing the array to remove non-binding ribosome display material from the arrayed target proteins,
- d) eluting from the arrayed target material any binding ribosome display material,
- e) amplifying the binding ribosome display material;
- and
- f) performing at least a further round of affinity selection repeating steps a)-d), whereby performing at least two rounds of affinity selection according to steps a) to d) yields bound ribosome display material having the highest affinity for at least one of the arrayed target proteins;
- and
- g) isolating pools of the bound ribosome display material to identify ribosome display material having the highest affinity for the at least one of the arrayed target proteins.
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Dependencies are inferred from the claim text.